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The brand new nitroxide slot sites with pyramid of gold revolutionary rule increased within the Intense 264.7 tissue stimulated with PMA versus handle tissues. That it rule try somewhat attenuated inside tissues pretreated that have telephone-impermeable SOD (Figure 1C). For each and every colour is short for wells checked to the some other months, appearing the brand new structure of data collected to the specific months and you can reproducibility of one’s overall performance round the time. The newest concentration of extracellular superoxide are influenced by deducting the fresh laws inside PMA muscle pretreated that have SOD in the signal after PMA from the lack of SOD (T).

  • Figure 1D illustrates the brand new calculation away from overall and you may extracellular superoxide.
  • Spin probes behave efficiently having ROS and produce a reliable nitroxide significant which are detected that have EPR.
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